Acoustophoretic Synchronization of Mammalian Cells in Microchannels

作者：Thevoz Patrick; Adams Jonathan D; Shea Herbert; Bruus Henrik; Soh H Tom^*

来源：Analytical Chemistry, 2010, 82(7): 3094-3098.

DOI：10.1021/ac100357u

We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(I) phase synchrony (similar to 84%) and throughput (3 x 10(6) cells/h per microchannel).

• 出版日期2010-4-1