Genetically modified organism (GMO) detection by biosensor based on SWCNT material

作者：Phuong Dinh Tam^*

来源：Current Applied Physics, 2015, 15(3): 397-401.

DOI：10.1016/j.cap.2015.01.017

• Microelectrodes
• Transgenic
• Roundup ready soybean
• Carbon nanotubes
• SWCNT-FET

This paper reports single-walled carbon nanotube-based biosensors for genetically modified organism (GMO) detection. Electrochemical electrode and single-walled carbon nanotube field effect transistor (SWCNT-FET)-based biosensors are used to determine the CaMV 35S promoter of Roundup Ready soybean. Given optimal conditions, both biosensors can effectively detect full complementarity with concentration as low as 1 nM. The sensitivity of the electrode-based biosensor is approximately 0.6 kU/nM while that of the SWCNT-FET-based biosensor is 0.32 nA/nM. Both biosensors were also used to determine a polymerase chain reaction-amplified sample. The obtained results showed that both sensors determined transgenic organisms well, thereby providing a useful tool for screening analysis of food samples.

• 出版日期2015-3
• 单位华中科技大学