摘要

The recent progress on bell pepper transformation through in planta approach is incredibly simple with high transformation efficiencies in indigenous varieties. This method produces chimeric primary transformants (T-0) hence, a large number of To plants and an efficient high throughput screening strategy is necessary to be developed. In the present investigation, we have standardized a rapid and highly efficient method of screening putative bell pepper transformants. Bell pepper transformants harbouring gfp genes was developed using in planta approach and 1050 etiolated T-1 seedlings were screened using fluorescent microscope to select GFP positive plants. The molecular analysis such as, gene specific PCRs, semiquantitative RT-PCR and Southern blots analysis corroborated the transgene integration in selected T-1 transformants. Based on progressive selection strategy, the transformation efficiency was 27.4% in gfp screened plants. The fluorescence based screening is viable, rapid, requires minimal labour, expense, and expertise with less percent of non-transformant escapes: further, this method can be extended from bell pepper to other crops to identify putative transformants developed through in planta approach.

  • 出版日期2011-7-27