科研之友
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摘要
OBJECTIVE: The objective of the study was to investigate changes in the expression of anglogenesis-related genes during the common terminal pathway of parturition including spontaneous labor at term, as well as preterm labor (PTL), induced by either bacteria or ovarlectomy.
STUDY DESIGN: Preterm pregnant mice (14.5 days of gestation) were treated with the following: (1) intrauterine injection of medlar (2) intrauterine injection of heat-inactivated Escherichia coli, (3) ovarlectomy and (4) sham operation. Tissues from mice at term (19.5 days of gestation) were collected at term not in labor, term in labor, and 12 hours postpartum. Anglogenesis-related gene expression levels were quantitated by the measurement of specific mRNAs in uterine tissue by RT-qPCR and analyzed by repeated-measures analysis of variance.
RESULTS: The following results were found: (1) microarray analysis of the uterine transcriptome indicated an enrichment for the gene ontol-ogy category of anglogenesis in bacteria-induced PTL samples (P <= .093) (2) several genes related to anglogenesis demonstrated significantly increased expression in samples in either term spontaneous labor or preterm labor and (3) qRT-PCR measurements demonstrated that spontaneous term labor and preterm labor induced by either bacteria or ovarlectomy all substantially increased the expression of multiple anglogenesis-related genes (P <= .0003 Angpt2, Ctgf, Cyr61, Dscr1, Pgf, Serpine1, Thbs1, and Wisp 1).
CONCLUSION: Spontaneous labor at term, as well as pathologically induced preterm labor, all result in greatly increased expression of anglogenesis-related genes in the uterus.
- 出版日期2008-5
