Accelerated bony defect healing based on chitosan thermosensitive hydrogel scaffolds embedded with chitosan nanoparticles for the delivery of BMP2 plasmid DNA

作者：Li, Hui; Ji, Qiuxia^*; Chen, Ximin; Sun, Yan; Xu, Quanchen; Deng, Panpan; Hu, Fang; Yang, Jianjun

来源：Journal of Biomedical Materials Research Part A, 2017, 105(1): 265-273.

DOI：10.1002/jbm.a.35900

• thermosensitive hydrogel
• chitosan nanoparticles
• tissue engineering scaffold
• BMP2
• endogenous repair

Regeneration of alveolar bone is among the main purposes of periodontal treatment. However, it is a challenge for both dentists and patients. An injectable chitosan-based thermosensitive hydrogel scaffold (CS/CSn-GP) was successfully designed and prepared with the incorporation of bone morphogenetic protein-2 plasmid DNA (pDNA-BMP2)-loaded chitosan nanoparticles (CS/CSn(pDNA-BMP2)-GP) in this study. The aim of this study was to evaluate the regenerative effect of this scaffold on the endogenous repair of alveolar bone. Thermosensitive properties, SEM scans and in vivo inflammatory reactions and degradation were evaluated. The CS/CSn-GP solution was initially injected into the muscle pouches of rats, where it changed into a gel, resulting in non-specific inflammation. It effectively enhanced new bone formation in rat calvarial defects and enhanced bony defect healing in beagle dogs. All results indicated that the CS/CSn(pDNA-BMP2)-GP complex system is a potential candidate as a targeted, multi-effect scaffold for the endogenous repair of alveolar bone.

• 出版日期2017-1
• 单位青岛大学; 山东大学