A two-stage enzymatic process for synthesis of extremely pure high oleic glycerol monooleate

作者：Zhu Qisi; Li Tie; Wang Yonghua^*; Yang Bo; Ma Yongjun

来源：Enzyme and Microbial Technology, 2011, 48(2): 143-147.

DOI：10.1016/j.enzmictec.2010.10.009

• Glycerol monooleate
• Enzymatic glycerolysis
• Binary solvent medium
• High oleic sunflower oil

This paper presents a research interest concentrating on aims to establish a feasible industrial process for enzymatic production of highly pure glycerol monooleate (GMO). The synthesis of high oleic glycerol monooleate by enzymatic glycerolysis of high oleic sunflower oil, using Novozyme 435 as the biocatalyst, in a binary solvent mixture of tert-butanol and tert-pentanol (80/20, v/v), at a lab-scale has been studied. A yield of 75.31% monoacylglycerol has been achieved at the first stage. A yield of 93.3% GMO was finally reached after further purification at the second stage. To evaluate the possibility of the process for industrialization, production of GMO was performed at a pilot-plant scale under the correspondingly adjusted conditions. A yield of 68.17% and 93.4% of GMO was obtained, respectively, at the end of the three stages.

• 出版日期2011-2-8
• 单位华南理工大学