Development of a Biochemical Oxygen Demand Sensor Using Gold-Modified Boron Doped Diamond Electrodes

作者：Ivandini Tribidasari A^*; Saepudin Endang; Wardah Habibah; Harmesa; Dewangga Netra; Einaga Yasuaki

来源：Analytical Chemistry, 2012, 84(22): 9825-9832.

DOI：10.1021/ac302090y

Gold-modified boron doped diamond (BDD) electrodes were examined for the amperometric detection of oxygen as well as a detector for measuring biochemical oxygen demand (BOD) using Rhodotorula mucilaginosa UICC Y-181. An optimum potential of -0.5 V (vs Ag/AgCl) was applied, and the optimum waiting time was observed to be 20 min. A linear calibration curve for oxygen reduction was achieved with a sensitivity of 1.4 mu A mg(-1) L oxygen. Furthermore, a linear calibration curve in the glucose concentration range of 0.1-0.5 mM (equivalent to 10-50 mg L-1 BOD) was obtained with an estimated detection limit of 4 mg L-1 BOD. Excellent reproducibility of the BOD sensor was shown with an RSD of 0.9%. Moreover, the BOD sensor showed good tolerance against the presence of copper ions up to a maximum concentration of 0.80 mu M (equivalent to 50 ppb). The sensor was applied to BOD measurements of the water from a lake at the University of Indonesia in Jakarta, Indonesia, with results comparable to those made using a standard method for BOD measurement.

• 出版日期2012-11-20