Activation transcription factor 1 involvement in the regulation of murine H-2D(d) expression

作者：Ishiguro N; Brown GD; Meruelo D

来源：Journal of Biological Chemistry, 1997, 272(25): 15993-16001.

DOI：10.1074/jbc.272.25.15993

Resistance to radiation leukemia virus-induced leukemia is correlated with an increase in H-2D expression on the thymocyte surface, Recently, it has been shown that elevated H-2D(d) expression on the infected thymocyte is a result of elevated mRNA transcription and that the transcriptional increase is correlated with elevated levels of a DNA binding activity, H-2 binding factor 1 (H-2 BF1), which recognizes the 5'-flanking sequences (5'-TGACGCG-3') of the H-2D(d) gene, This target for transcription factor binding has been found to be identical in the 5'-regulatory region of 12 rodent class I genes, nine of which have been shown to be functional genes, Furthermore, tills cis-element is found 5' of 20 primate class I genes (15 human genes), seven of which are known to be functional, Here, we demonstrate that activation transcription factor 1 (ATF-1) is one component of H-2 BF1, In addition, the levels of ATF-1 mRNA in uninfected and radiation leukemia virus-infected thymocytes parallel those of H-2D(d) mRNA, and therefore, it is suggested that ATF-1 up-regulates the transcription of the H-2D(d) gene after radiation leukemia virus infection of thymocytes. Transfection experiments also demonstrate that ATF-1 activates a reporter plasmid that contains the H-2 BF1 motif, but not a reporter lacking this motif, This is the first demonstration of the interaction of ATF-1 with 5'-regulatory sequences of major histocompatibility complex class I genes.

• 出版日期1997-6-20