AtSERK1 expression precedes and coincides with early somatic embryogenesis in Arabidopsis thaliana

作者：Salaj Jan^*; von Recklinghausen Iris R; Hecht Valerie; de Vries Sacco C; Schel Jan H N; van Lammeren Andre A M

来源：Plant Physiology and Biochemistry, 2008, 46(7): 709-714.

DOI：10.1016/j.plaphy.2008.04.011

• antibodies
• Arabidopsis
• AtSERK1
• GUS
• SERK
• somatic embryogenesis
• suspension culture

The Arabidopsis thaliana primordia timing (pt) mutant was transformed with an AtSERK1::GUS construct. Liquid cultures of this line were used to study the relationship between somatic embryogenesis and the expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (AtSERK1) as a marker for cells competent to form embryos. In order to search for the expression of AtSERKI::GUS during early stages of somatic embryogenesis, histochemical as well as immunochemical approaches were used for the detection of beta-glucuronidase (GUS). Four sites of AtSERKI expression were found in the embryogenic cultures: in embryogenic callus, where primary somatic embryos developed; in the basal parts of primary somatic embryos; in the outer layers of cotyledons of primary somatic embryos where secondary embryos were formed; and in provascular and vascular strands of developing somatic embryos. The in vitro expression of AtSERKI::GUS coincides with embryogenic development up to the heart-shaped stage. Prior to the expression in embryos, AtSERK1 was expressed in single cells and small cell clusters, indicating that AtSERK1 indeed marks embryogenic competence. Its expression in (pro)vascular strands, suggests that embryogenic cells in tissue culture retain at least in part their original identity.

• 出版日期2008-7