Adipose tissue transcriptome changes during obesity development in female dogs

作者：Grant Ryan W; Boler Brittany M Vester; Ridge Tonya K; Graves Thomas K; Swanson Kelly S^*

来源：Physiological Genomics, 2011, 43(6): 295-307.

DOI：10.1152/physiolgenomics.00190.2010

• gene expression
• metabolism
• extracellular matrix
• oxidation

Grant RW, Vester Boler BM, Ridge TK, Graves TK, Swanson KS. Adipose tissue transcriptome changes during obesity development in female dogs. Physiol Genomics 43: 295-307, 2011. First published January 11, 2011; doi: 10.1152/physiolgenomics.00190.2010.-During the development of obesity, adipose tissue undergoes major expansion and remodeling, but the biological processes involved in this transition are not well understood. The objective of this study was to analyze global gene expression profiles of adipose tissue in dogs, fed a high-fat diet, during the transition from a lean to obese phenotype. Nine female beagles (4.09 +/- 0.64 yr; 8.48 +/- 0.35 kg) were randomized to ad libitum feeding or body weight maintenance. Subcutaneous adipose tissue biopsy, blood, and dual x-ray absorptiometry measurements were collected at 0, 4, 8, 12, and 24 wk of feeding. Serum was analyzed for glucose, insulin, fructosamine, triglycerides, free fatty acids, adiponectin, and leptin. Formalin-fixed adipose tissue was used for determination of adipocyte size. Adipose RNA samples were hybridized to Affymetrix Canine 2.0 microarrays. Statistical analysis, using repeated-measures ANOVA, showed ad libitum feeding increased (P < 0.05) body weight (0 wk, 8.36 +/- 0.34 kg; 24 wk, 14.64 +/- 0.34 kg), body fat mass (0 wk, 1.36 +/- 0.24 kg; 24 wk, 6.52 +/- 0.24 kg), adipocyte size (0 wk, 114.66 +/- 17.38 mu m(2); 24 wk, 320.97 +/- 0.18.17 mu m(2)), and leptin (0 wk, 0.8 +/- 1.0 ng/ml; 24 wk, 12.9 +/- 1.0 ng/ml). Microarrays displayed 1,665 differentially expressed genes in adipose tissue as weight increased. Alterations were seen in adipose tissue homeostatic processes including metabolism, oxidative stress, mitochondrial homeostasis, and extracellular matrix. Adipose transcriptome changes highlight the dynamic and adaptive response to ad libitum feeding and obesity development.

• 出版日期2011-3