An experimental test of stroke recovery by implanting a hyaluronic acid hydrogel carrying a Nogo receptor antibody in a rat model

作者：Ma, Jun; Tian, Wei-Ming; Hou, Shao-Ping; Xu, Qun-Yuan; Spector, Myron; Cui, Fu-Zhai^*

来源：Biomedical Materials (Bristol), 2007, 2(4): 233-240.

DOI：10.1088/1748-6041/2/4/005

• CEREBRAL-ARTERY OCCLUSION
• NEURITE GROWTH-INHIBITORS
• SPINAL-CORD
• AXONAL REGENERATION
• FUNCTIONAL RECOVERY
• PLASTICITY
• ADULT
• BRAIN
• BLOCKADE
• DELIVERY

The objective of the study was to determine the effects of a hyaluronic-acid-based (HA-based) hydrogel implant, carrying a polyclonal antibody to the Nogo-66 receptor (NgR), on adult rats that underwent middle cerebral artery occlusion (MCAO). Behavioral tests of a forelimb-reaching task suggested that the disabled function of the impaired forelimb in this stroke model was ameliorated by the implant to a certain extent. These behavioral findings were correlated with immunohistochemical results of investigating the distribution of NgR antibody, neurofilaments (NF) and neuron-specific class III beta-tubulin (TuJ1) in the brain sections. The porous hydrogel functioned as a scaffold to deliver the NgR antibody, support cell migration and development. In addition, it was found NF-positive and TuJ1-positive expressions were distributed in the implanted hydrogel. Collectively, the results demonstrate the promise of the HA hydrogel as a scaffold material and the delivery vehicle of the NgR antibody for the repair of defects and the support of neural regeneration in the brain.

• 出版日期2007-12
• 单位首都医科大学; 清华大学; 北京市神经外科研究所