Multicentric Determination of Reference Ranges for Automated Blood Counts

作者：Nebe Thomas^*; Bentzien Frank; Bruegel Mathias; Fiedler Georg Martin; Gutensohn Kai; Heimpel Hermann; Krebs Nicole; Ossendorf Manfred; Peter Schuff Werner; Stamminger Gudrun; Baum Hannsjoerg

来源：Journal of Laboratory Medicine, 2011, 35(1): 3-28.

DOI：10.1515/JLM.2011.004

• blood differential
• complete blood count
• hematology
• hematology analyzer
• reference ranges
• reticulocytes

The analysis of blood cell count including differential, is the most frequent laboratory test and has still essential diagnostic value in the diagnosis of primary or secondary diseases of the hematopoietic system. The interpretation is based on a comparison with age matched reference intervals, despite the fact that a comparison with the patients own preceding values allows a more precise statement. In the present study, blood counts and differential were performed in 1158 healthy males and females with an age ranging from 16 to 75 years were investigated using current hematology systems. The donors were examined by personnel physicians and those examining blood donors in transfusion medicine and in addition were asked via standardized questionnaire. The study resulted in reference ranges for the complete blood count, the differential blood count and reticulocytes. For several analytes, age-, gender- or analyzer-specific reference ranges were obtained. We found an age dependent decrease of the erythrocyte concentration and an increase for MCH and MCV as well as for basophils. These findings could be important for the dignosis of diseases of the elderly like myeloproliferative diseases (MPN) and myelodysplasias (MDS). Gender specific changes could be shown for the hemoglobulin-, the erythrocyte- and reticulocyte concentration, as well as for hematocrit and MCHC, being reduced in females. On the other hand, females showed significant higher levels for platelets and leukocytes, based on an increase of neutrophils and lymphocytes. The leukocytosis of smokers was confirmed for neutrophils and lymphocytes but lower than one could expect from recent studies. A multicentric study shows a broader distribution compared to unicentric data but this probably better reflects the reality and improves the applicability of the reference ranges.

• 出版日期2011