Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation

作者：Segovia Yolanda^*; Victory Noemi; Peinado Irene; Garcia Valverde Laura M; Garcia Magdalena; Aizpurua Jon; Monzo Ana; Jose Gomez Torres Maria

来源：Journal of Reproduction and Development, 2017, 63(4): 377-382.

DOI：10.1262/jrd.2017-009

• Cryopreservation
• Electron microscopy
• In vitro maturation

The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MR oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM). Each oocyte presented a rounded shape, with an intact oolemma, and was surrounded by a continuous zona pellucida and perivitelline space. Statistical analysis comparing oocytes vitrified before or after IVM indicated that there were no significant differences between examined characteristics.

• 出版日期2017-8