科研之友
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摘要
A serological surveillance was carried Out using an enzyme-linked immunosorbent assay(ELISA)for investigating the situation of ovine Theileriosis in China.During 2004 to 2005.the total 1 170 sera samples of sheep and goats were collected from some provinces in China,which include 13 counties in 5 provinces,and detected the anti-theileria antibody by the ELISA.The results of detection indicated that ovine Theileriosis had prevalent in 13 counties in 5 provinces but not Jingtai county,the highest naturally infested positive rate of theileria was 93.8% in Lintao,which suggested that ovine Theileriosis was seriously in China.In addition,the sera were collected from three sheep experimentally infected ovine Theileriosis by Haemaphyslia qinghaiensis in the field during April 2001 to March 2002,were used to evaluate the annual kinetics of average values of anti-theileria antibody.The kinetics showed that anti-theileri antibody kept in higher level in 40 d after infestation.It is concluded that the optimal time for sera collection is mid-May when the ticks emerged in early of April in the field.
- 出版日期2009
- 单位中国农业科学院; 中国农业科学院兰州兽医研究所
