A fluorescent DNA based probe for Hg(II) based on thymine-Hg(II)-thymine interaction and enrichment via magnetized graphene oxide

作者：Li, Meng-Ke; Hu, Liu-Yin; Niu, Cheng-Gang^*; Huang, Da-Wei^*; Zeng, Guang-Ming

来源：Microchimica Acta, 2018, 185(3): 207.

DOI：10.1007/s00604-018-2689-6

• Naphthalimide
• Magnetic enrichment
• Quencher
• Fluorescent labeling
• Amplify
• Hybridization
• Nanoparticles
• Mercury ions

The authors describe a fluorometric assay for the determination of Hg(II). A naphthalimide derivative is used as a label for a thymine (T) rich ssDNA, and graphene oxide magnetized with Fe3O4 nanoparticles acts as a quencher and preconcentrators. In the absence of Hg(II), the labeled ssDNA does not separate from the magnetized graphene oxide. As a result, fluorescence is fully quenched. In the presence of Hg(II), a T-Hg(II)-T link is formed dues to the highly affinity between T and Hg(II). Hence, fluorescence is restored. The assay has a linear response in the 1.0 to 10.0 nM Hg(II) concentration range, and a 0.65 nM detection limit. The method is selective and sensitive. It was applied to the analysis of spiked environmental water samples, and data agreed well with those obtained by atomic fluorescence spectrometry.

• 出版日期2018-3
• 单位湖南大学; 环境保护部华南环境科学研究所