A magnetic electrochemical immunosensor for the detection of phosphorylated p53 based on enzyme functionalized carbon nanospheres with signal amplification

作者：Luo, Yanan; Asiri, Abdullah Mohamed; Zhang, Xiao; Yang, Guohai; Du, Dan^*; Lin, Yuehe

来源：RSC Advances, 2014, 4(96): 54066-54071.

DOI：10.1039/c4ra10516h

• GRAPHENE OXIDE
• PROTEIN
• NANOPARTICLES
• NANOTUBES
• CELL
• EXPRESSION
• ELECTRODES

Protein phosphorylation plays an important role in many biological processes and might be used as a potential biomarker in clinical diagnoses. We reported the development of a nanomaterial enhanced disposable immunosensor for ultrasensitive detection of phosphorylated p53 at Ser392 (phospho-p53(392)) using enzyme functionalization of carbon nanospheres (CNSs) as a signal amplification label and magnetic beads (MB) coupled with screen printed carbon electrodes as electrochemical transducers. In this work, horseradish peroxidase (HRP) and phospho-p53(392) detection antibody (Ab(2)) were co-linked to CNSs (HRP-CNSs-Ab(2)) for signal amplification, and functionalized MB was used as a platform to capture a large amount of primary antibodies (Ab(1)). The proposed signal amplification strategy with a sandwich-type immunoreaction significantly enhanced the sensitivity of detection of biomarkers. Under optimal conditions, the immunosensor had a highly linear voltammetric response to the phospho-p53(392) concentration in the range of 0.01 to 5 ng mL(-1), with a detection limit of 3.3 pg mL(-1). The results provided great potential for point-of-care detection of other phosphorylated proteins and clinical applications.

• 出版日期2014
• 单位华中师范大学