科研之友
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摘要
Background: Children cared for outside the home are at an increased risk of enteric disease. Microbiological analyses were performed on environmental samples collected from child care facilities in North and South Carolina. %26lt;br%26gt;Methods: There were 326 samples collected from 40 facilities corresponding to common surfaces (77% of samples) and the hands of care providers (23% of samples). Samples were analyzed for total aerobic plate counts (APCs), total coliforms, biotype I Escherichia coli, and pathogens Shigella spp, Salmonella enterica, E coli O157, Campylobacter jejuni, and human norovirus. %26lt;br%26gt;Results: Median APCs and coliform counts for hands were 4.6 and 1.0 log(10) colony-forming units (CFU) per hand, respectively. Median APCs for surfaces were 2.0 and 2.6 log(10) CFU for flat and irregular surfaces, respectively. Coliforms were detected in 16% of samples, with counts ranging from 1.0 log(10) to %26gt;4.3 log(10) CFU, with higher counts most often observed for hand rinse samples. Biotype I E coli counts were below assay detection limits (%26lt;1 log(10) CFU) for all but 1 sample. No samples were positive for any of the 4 bacterial pathogens, whereas 4 samples showed evidence of human norovirus RNA. %26lt;br%26gt;Conclusion: The relative absence of pathogens and biotype I E coli in environmental samples suggests the child care facilities sampled in this study managed fecal contamination well.
- 出版日期2014-10
