Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients

作者：Ali Aisyah; Vasudevan Ramachandran; Ismail Patimah^*; Seong Christoper Lim Thiam; Chakravarthi Srikumar

来源：Journal of the Renin-Angiotensin-Aldosterone System, 2015, 16(4): 1337-1343.

DOI：10.1177/1470320310392096

• Angiotensin converting enzyme
• end-stage renal disease
• insertion
• deletion
• PCR
• polymorphism
• Malaysia

Introduction: Insertion/deletion (I/D) polymorphisms found in the angiotensin converting enzyme (ACE) gene have been associated with hypertension, diabetes and renal disease. The present study sought to determine the association of I/D polymorphisms of the ACE gene with end-stage renal disease (ESRD) patients in Malaysia.
Materials and methods: A total of 380 subjects were recruited to determine the genotypes of I/D polymorphisms of the ACE gene. Genotyping was performed using a PCR method. Statistical analyses were carried out using statistical software, and a level of p < 0.05 was considered statistically significant.
Results: The frequencies for II, ID and DD genotypes of the ACE gene were 24.7%, 65.80% and 9.47%, respectively, in ESRD patients, and in control subjects were 45.26%, 47.37% and 7.37% respectively. The frequency for the D allele was found to be higher (42.40%) in ESRD patients compared to control subjects (31.05%). The genotypic and allelic frequencies of I/D polymorphisms of the ACE gene differed significantly (p < 0.05) between ESRD patients and control subjects in the Malaysian population.
Conclusion: The findings of this study indicate that I/D polymorphisms of the ACE gene are a useful marker and are likely to play a major role in determining genetic susceptibility to ESRD in the Malaysian population.

• 出版日期2015-12