Agitated shoot cultures of Aronia arbutifolia and Aronia x prunifolia: biotechnological studies on the accumulation of phenolic compounds and biotransformation capability

作者：Szopa Agnieszka^*; Kubica Pawe; Ekiert Halina

来源：Plant Cell, Tissue and Organ Culture, 2018, 134(3): 467-479.

DOI：10.1007/s11240-018-1436-3

• Flavonoids
• Phenolic acids
• Arbutin
• Hydroquinone
• Red Aronia
• Purple Aronia

In methanolic extracts of the biomass from agitated cultures of Aronia arbutifolia and Aronia x prunifolia grown on four variants of the Murashige and Skoog (MS) medium, with different concentrations of plant growth regulators (PGRs): BA and NAA (0.5-3.0 mg/l), the quantities of phenolic acids (19 compounds) and flavonoids (11 compounds) were estimated using the LC-DAD method. The amounts of individual metabolites and total contents were dependent on the concentration of PGRs in MS medium variant. The maximum total amounts of phenolic acids and flavonoids reached 360.80 and 65.26 mg/100 g DW, and 659.51 and 78.34 mg/100 g DW for A. arbutifolia and A. x prunifolia, respectively. The main metabolites in the biomass of both plants were chlorogenic acid, rosmarinic acid and quercitrin (max. 175.94, 147.98 and 41.14 mg/100 g DW, and 260.34, 225.26 and 78.34 mg/100 g DW, respectively). The cells of both plants convert the exogenously supplied hydroquinone into its beta-D-glucoside-arbutin. The maximal total content of the product accumulated in the biomass and media reached 83.55 and 73.62 mg/g DW. The obtained results demonstrated for the first time a high biosynthetic potential of agitated cultures of both plants.

• 出版日期2018-9