alpha-Mangostin Improves Glucose Uptake and Inhibits Adipocytes Differentiation in 3T3-L1 Cells via PPAR gamma, GLUT4, and Leptin Expressions

作者：Taher Muhammad^*; Amiroudine Mohamed Zaffar Ali Mohamed; Zakaria Tengku Muhamad Faris Syafiq Tengku; Susanti Deny; Ichwan Solachuddin J A; Kaderi Mohd Arifin; Ahmed Qamar Uddin; Zakaria Zainul Amiruddin

来源：Evidence-Based Complementary and Alternative Medicine, 2015, 2015: 740238.

DOI：10.1155/2015/740238

Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of alpha-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPAR gamma, GLUT4, and leptin expressions. alpha-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 mu M of alpha-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[H-3] glucose uptake activity showed that alpha-mangostin significantly improved the glucose uptake (P < 0.05) with highest activity found at 25 mu M. In addition, alpha-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 mu M of alpha-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPAR gamma gene expression. Induction of glucose uptake and free fatty acid release by alpha-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that alpha-mangostin might be possible candidate for the effective management of obesity in future.

• 出版日期2015