A PNA microarray for tomato genotyping

作者：Tedeschi Tullia; Calabretta Alessandro; Bencivenni Mariangela; Manicardi Alex; Corrado Giandomenico; Caramante Martina; Corradini Roberto; Rao Rosa; Sforza Stefano^*; Marchelli Rosangela

来源：Molecular Biosystems, 2011, 7(6): 1902-1907.

DOI：10.1039/c1mb05048f

The design and development of a PNA microarray designed for the simultaneous identification of several SNPs characteristic of seven different tomato varieties is described. Highly selective arginine-based monomer containing PNAs (Arg-PNAs) have been used in order to obtain very selective probes. Seven modified PNA probes were synthesised and their binding properties in solution were studied. PNA-microarrays based on these probes were prepared and applied to SNP discrimination in model experiments using oligonucleotide mixtures simulating the different sequences of the seven tomato varieties. The strength and the limitations of such a system for SNP recognition are thoroughly discussed.

• 出版日期2011