Ultrasensitive detection of ATP based on ATP regeneration amplification and its application in cell homogenate and human serum

作者：Guo, Yingshu^*; Sun, Xiaofeng; Yang, Guangxu; Liu, Jia

来源：Chemical communications, 2014, 50(57): 7659-7662.

DOI：10.1039/c4cc01458h

• ROLLING CIRCLE AMPLIFICATION
• SIGNAL AMPLIFICATION
• LABEL-FREE
• ADENOSINE-TRIPHOSPHATE
• FLUORESCENT DETECTION
• EXONUCLEASE III
• SMALL MOLECULES
• DNA
• ASSAY
• PROTEINS

A conformation-switching aptamer molecule that could be circularized without ligation DNA was designed. Pyrophosphate (PPi) was converted to ATP, resulting in higher signals for ATP detection. Meanwhile, the method has significant implications for real applications.

• 出版日期2014
• 单位青岛科技大学