A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells

作者:Sherman Jonathan H*; Redpath Gerard T; Redick Jan A; Purow Benjamin W; Laws Edward R; Jane John A Jr; Shaffrey Mark E; Hussaini Isa M
来源:Journal of Neuroscience Methods, 2011, 198(1): 99-102.
DOI:10.1016/j.jneumeth.2011.03.003

摘要

Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1.4-benzoquinone) for immunoflourescence staining of neurospheres.

  • 出版日期2011-5-15