Advances in methods for identification and characterization of plant transporter function

作者：Larsen Bo; Xu Deyang; Halkier Barbara Ann; Nour Eldin Hussam Hassan^*

来源：Journal of Experimental Botany, 2017, 68(15): 4045-4056.

DOI：10.1093/jxb/erx140

• Exporter identification
• expression cloning
• forward genetics transporter identification
• in silico approaches for transporter identification
• metabolic engineering-based transporter screen
• Nitrate and Peptide transporter Family (NPF)
• plant transporter identification
• receptor-and sensor-based screen
• transporter substrate specificity
• Xenopus oocytes

Transport proteins are crucial for cellular function at all levels. Numerous importers and exporters facilitate transport of a diverse array of metabolites and ions intra-and intercellularly. Identification of transporter function is essential for understanding biological processes at both the cellular and organismal level. Assignment of a functional role to individual transporter proteins or to identify a transporter with a given substrate specificity has notoriously been challenging. Recently, major advances have been achieved in function-driven screens, phenotype-driven screens, and in silico-based approaches. In this review, we highlight examples that illustrate how new technology and tools have advanced identification and characterization of plant transporter functions.

• 出版日期2017-7-10