A bioluminescent Pseudomonas aeruginosa wound model reveals increased mortality of type 1 diabetic mice to biofilm infection

作者：Hunt A M Agostinho; Gibson J A; Larrivee C L; O'Reilly S; Navitskaya S; Needle D B; Abramovitch R B; Busik J V; Waters C M^*

来源：Journal of Wound Care, 2017, 26(7): S24-S33.

DOI：10.12968/jowc.2017.26.Sup7.S24

• wound healing
• biofilm
• infection
• Pseudomonas aeruginosa
• cyclic di-GMP

Objective: To examine how bacterial biofilms, as contributing factors in the delayed closure of chronic wounds in patients with diabetes, affect the healing process.
Method: We used daily microscopic imaging and the IVIS Spectrum in vivo imaging system to monitor biofilm infections of bioluminescent Pseudomonas aeruginosa and evaluate healing in non-diabetic and streptozotocin-induced diabetic mice.
Results: Our studies determined that diabetes alone did not affect the rate of healing of full-depth murine back wounds compared with nondiabetic mice. The application of mature biofilms to the wounds significantly decreased the rate of healing compared with non-infected wounds for both non-diabetic as well as diabetic mice. Diabetic mice were also more severely affected by biofilms displaying elevated pus production, higher mortality rates and statistically significant increase in wound depth, granulation/fibrosis and biofilm presence. Introduction of a mutant Pseudomonas aeruginosa capable of producing high concentrations of cyclic di-GMP did not result in increased persistence in either diabetic or non-diabetic animals compared with the wild type strain.
Conclusion: Understanding the interplay between diabetes and biofilms may lead to novel treatments and better clinical management of chronic wounds.

• 出版日期2017-7