New microsatellite markers for the assessment of fine-scale dispersal patterns in the endangered montane caddisfly Drusus discolor

作者：Geismar Jutta; Sauer Jan; Haase Peter; Nowak Carsten^*

来源：Conservation Genetics Resources, 2011, 3(4): 605-607.

DOI：10.1007/s12686-011-9414-7

• Short tandem repeats
• Landscape genetics
• Dispersal
• Aquatic insects
• Microsatellite development
• Montane biodiversity
• Global climate change

We present a panel of variable microsatellite markers for the montane caddisfly Drusus discolor, a rare species that we use as a model for the assessment of climate change effects on the montane freshwater community. 454 sequencing technology was used in order to generate random sequence data. Of a total of 2,648 microsatellite-carrying fragments identified, 11 variable, reproducible markers were generated and tested on 41 individuals from two populations. Allelic diversity ranged from 3 to 10 alleles and heterozygosity values varied from 0 to 0.89 (H (O)) and 0.12 to 0.86 (H (E)). A cross-species test on four caddisfly species showed that a subset of markers amplifies in related taxa.

• 出版日期2011-10
• 单位国家气候中心