Molecular transport through large-diameter DNA nanopores

作者：Krishnan Swati; Ziegler Daniela; Arnaut Vera; Martin Thomas G; Kapsner Korbinian; Henneberg Katharina; Bausch Andreas R; Dietz Hendrik; Simmel Friedrich C^*

来源：Nature Communications, 2016, 7(1): 12787.

DOI：10.1038/ncomms12787

DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing and synthetic biology. Here we introduce a large DNA membrane channel with an approximate to 4 nm diameter pore, which has stable electrical properties and spontaneously inserts into flat lipid bilayer membranes. Membrane incorporation is facilitated by a large number of hydrophobic functionalizations or, alternatively, streptavidin linkages between biotinylated channels and lipids. The channel displays an Ohmic conductance of approximate to 3 nS, consistent with its size, and allows electrically driven translocation of single-stranded and double-stranded DNA analytes. Using confocal microscopy and a dye influx assay, we demonstrate the spontaneous formation of membrane pores in giant unilamellar vesicles. Pores can be created both in an outside-in and an inside-out configuration.

• 出版日期2016-9