New genetic polymorphisms of KiSS-1 gene and their association with litter size in goats

作者：Hou, J. X.; An, X. P.; Wang, J. G.; Song, Y. X.; Cui, Y. H.; Wang, Y. F.; Chen, Q. J.; Cao, B. Y.^*

来源：Small Ruminant Research, 2011, 96(2-3): 106-110.

DOI：10.1016/j.smallrumres.2010.11.013

• PCR-SSCP
• Base deletions
• SNP
• Candidate gene

In the present study, the polymorphisms of kisspeptin (KiSS-1) gene were analyzed in 652 individuals from three goat breeds: Xinong Saanen goats (SG), Guanzhong goats (GZ) and Boer goats (BG). Three alleles (T, C and G) and four genotypes (Tf, TC, CC and TG) were detected in the intron 2 of KISS-1 gene by PCR-SSCP analysis. In addition, two single nucleotide polymorphisms (SNPs) - T2643C and 8bp base deletions (2677ACTTCCCC) were identified by DNA sequencing. The SNPs loci were in Hardy-Weinberg disequilibrium in three goat breeds (P < 0.05). After comparing genotypic distribution within three goat breeds, BC had conspicuous differences from SG and GZ (P < 0.001). Association of polymorphism with litter size was done in SG and GZ breeds, which showed to be associated with litter size in both goat breeds. The SNP in goat KISS-1 gene had significant effects on litter size (P < 0.05). Therefore, these results suggest that KISS-1 gene is a strong candidate gene that affects litter size in goats.

• 出版日期2011-4
• 单位西北农林科技大学