Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

作者：Krcmova Lenka^*; Solichova Dagmar; Melichar Bohuslav; Kasparova Marketa; Plisek Jiri; Sobotka Lubos; Solich Petr

来源：Talanta, 2011, 85(3): 1466-1471.

DOI：10.1016/j.talanta.2011.06.027

• Neopterin
• Kynurenine
• Tryptophan
• HPLC
• Monolithic column
• Clinical research

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm x 50 mm + 3.0 mm x 100 mm and 4.6 x 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.

• 出版日期2011-9-15