An efficient quantitation method of next-generation sequencing libraries by using MiSeq sequencer

作者：Katsuoka Fumiki^*; Yokozawa Junji; Tsuda Kaoru; Ito Shin; Pan Xiaoqing; Nagasaki Masao; Yasuda Jun; Yamamoto Masayuki

来源：Analytical Biochemistry, 2014, 466: 27-29.

DOI：10.1016/j.ab.2014.08.015

• Next-generation sequencing
• Library quantitation
• Library QC

Library quantitation is a critical step to obtain high data output in Illumina HiSeq sequencers. Here, we introduce a library quantitation method that uses the Illumina MiSeq sequencer designated as quantitative MiSeq (qMiSeq). In this procedure, 96 dual-index libraries, including control samples, are denatured, pooled in equal volume, and sequenced by MiSeq. We found that relative concentration of each library can be determined based on the observed index ratio and can be used to determine HiSeq run condition for each library. Thus, qMiSeq provides an efficient way to quantitate a large number of libraries at a time.

• 出版日期2014-12-1