DETERMINATION OF VITAMIN A IN INFANT MILK-BASED FORMULAS AND PHARMACEUTICAL FORMULATIONS USING FLOW INJECTION WITH Ce(IV)-Na2SO3 CHEMILUMINESCENCE DETECTION

作者：Rishi Lubna; Yaqoob Mohammad^*; Asghar Mohammad; Shah S Haider; Nabi Abdul

来源：Analytical Letters, 2012, 45(14): 2037-2052.

DOI：10.1080/00032719.2012.680053

• Chemiluminescence
• Flow injection analysis
• Infant formula powder and pharmaceutical formulations
• Vitamin A

A rapid and simple flow injection (FI) method is reported for the determination of vitamin A (retinol) based on its strong enhancing effect on the Ce(IV)-Na2SO3 chemiluminescence (CL) reaction in an acidic solution. The effect of key chemical and physical parameters (i.e., reagent concentrations, flow rate, and sample volume) was optimized and potential interferences examined. Under the selected experimental conditions, a linear calibration was obtained between the CL intensity and vitamin A concentration in the range 0.1-8.0 mu g mL(-1) (r(2) = 0.9986, n = 8). The limit of detection (3 s x blank) was 0.01 mu g mL(-1) retinol (n = 6) and the relative standard deviation (RSD) for 0.25 mu g mL(-1) retinol was 2.3% (n = 10) with a sampling rate of 180 h(-1). The method was successfully applied to infant milk-based formulas and pharmaceutical formulations and the results were not significantly different at 95% confidence interval with those obtained by using a spectrophotometric reference method. The possible CL mechanism is also discussed briefly supporting with UV-visible, fluorescence, and CL spectra.

• 出版日期2012