Analysis of sulfonamides, tilmicosin and avermectins residues in typical animal matrices with multi-plug filtration cleanup by liquid chromatography-tandem mass spectrometry detection

作者：Qin Yuhong; Jatamunua Freedom; Zhang Jingru; Li Yanjie; Han Yongtao; Zou Nan; Shan Jihao; Jiang Yanbin; Pan Canping^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2017, 1053: 27-33.

DOI：10.10164/j.jchromb.2017.04.006

• Veterinary drugs
• Residues analysis
• Animal matrices
• Multi-plug filtration cleanup

The frequent use of various veterinary drugs could lead to residue bioaccumulation in animal tissues, which could cause dietary risks to human health. In order to quickly analyze the residues, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for detecting Sulfonamides, Tilmicosin and Avermectins (AVMs) residues in animal samples. For sample preparation, modified QuEChERS (quick, easy, cheap, effective, rugged and safe) and ultrasound-assisted extraction (UAE) methods were used. For sample cleanup, n-Hexane delipidation and multi-plug filtration cleanup (m-PFC) method based on primary-secondary amine (PSA) and octadecyl-silica (C18) were used, followed by LC-MS/MS analysis. It was validated on 7 animal matrices (bovine, caprine, swine meat and their kidneys, milk) at two fortified concentration levels of 5 and 100 mu g/kg. The recoveries ranged from 82 to 107% for all analytes with relative standard deviations (RSDs) less than 15%. Matrix-matched calibrations were performed with coefficients of determination above 0.998 for all analytes within concentration levels of 5-500 mu g/kg. The developed method was successfully used to analysis veterinary drugs of real animal samples from local markets.

• 出版日期2017-5-15
• 单位中国农业大学; 中国动物疫病预防控制中心; 中国农业科学院