A NEW CDC-GENE REQUIRED FOR S-PHASE ENTRY OF SCHIZOSACCHAROMYCES-POMBE ENCODES A PROTEIN SIMILAR TO THE CDC10+ AND SWI4-GENE PRODUCTS

作者：TANAKA K^*; OKAZAKI K; OKAZAKI N; UEDA T; SUGIYAMA A; NOJIMA H; OKAYAMA H

来源：The EMBO Journal, 1992, 11(13): 4923-4932.

• CDC10
• SWI6
• ANKYRIN MOTIF
• CELL CYCLE
• SCHIZOSACCHAROMYCES-POMBE
• START

We have isolated a new cell division cycle gene (res1+) required for entry into S phase, as a multicopy dual suppressor of the pat1 and cdc10 mutants of the fission yeast Schizosaccharomyces pombe. The res1+ gene specifies a 72 kDa protein with two copies of the cdc10/SWI6 motif. A disruptant of res1+ grows poorly at 30-degrees-C with severe heat- and cold-sensitivities, and completely arrests in G1 at 36-degrees-C and 23-degrees-C. The arrested disruptant retains a full conjugation ability. In addition to the cdc10/SWI6 motif, Res1 and SWI4 proteins share a remarkable homology in their amino-terminal region, whereas Cdc10 and SWI6 do so in their carboxy-terminal region. Moreover, the amino-terminal region is essential for the function of Res1 as it is for the function of SWI4. Furthermore, analogous to the relationship of SWI4 to SWI6, the res1+ gene effectively rescues cdc10 mutants, but the cdc gene cannot rescue the res1- phenotype. Thus, striking similarities exist in both structural and functional relationships between Res1 and SWI4, and between Cdc10 and SWI6. In view of the fact that SWI4 and SWI6 form a transcription factor complex and activate promoters containing the SWI4/SWI6 dependent cell-cycle box, Res1 might be a putative association partner of Cdc10 which appears to be involved at least in the activation of promoters containing a MluI cell-cycle box.

• 出版日期1992-12