Hide and seek: Identification and confirmation of small molecule protein targets

作者：Ursu Andrei; Waldmann Herbert

来源：Bioorganic & Medicinal Chemistry Letters, 2015, 25(16): 3079-3086.

DOI：10.1016/j.bmcl.2015.06.023

• Target identification
• Target confirmation
• Affinity chromatography
• Linker
• Chemical probes

Target identification and confirmation for small molecules is often the rate limiting step in drug discovery. A robust method to identify proteins addressed by small molecules is affinity chromatography using chemical probes. These usually consist of the compound of interest equipped with a linker molecule and a proper tag. Recently, methods emerged that allow the identification of protein targets without prior functionalization of the small molecule of interest. The digest offers an update on the newest developments in the area of target identification with special focus on confirmation techniques.

• 出版日期2015-8-15