Muscle and connective tissue progenitor populations show distinct Twist1 and Twist3 expression profiles during axolotl limb regeneration

作者：Kragl Martin; Roensch Kathleen; Nuesslein Ina; Tazaki Akira; Taniguchi Yuka; Tarui Hiroshi; Hayashi Tetsutaro; Agata Kiyokazu; Tanaka Elly M^*

来源：Developmental Biology, 2013, 373(1): 196-204.

DOI：10.1016/j.ydbio.2012.10.019

• Twist1
• Twist3
• Lateral plate mesoderm
• Presomitic mesoderm
• Single cell pcr
• Limb regeneration

Limb regeneration involves re-establishing a limb development program from cells within adult tissues. Identifying molecular handles that provide insight into the relationship between cell differentiation status and cell lineage is an important step to study limb blastema cell formation. Here, using single cell PCR, focusing on newly isolated Twist1 sequences, we molecularly profile axolotl limb blastema cells using several progenitor cell markers. We link their molecular expression profile to their embryonic lineage via cell tracking experiments. We use in situ hybridization to determine the spatial localization and extent of overlap of different markers and cell types. Finally, we show by single cell PCR that the mature axolotl limb harbors a small but significant population of Twist1(+) cells.

• 出版日期2013-1-1