Math1 Gene Transfer Based on the Delivery System of Quaternized Chitosan/Na-Carboxymethyl-beta-Cyclodextrin Nanoparticles

作者：Ren, Li-Li; Wu, Yan^*; Han, Dong; Zhao, Li-Dong; Sun, Quan-Mei; Guo, Wei-Wei; Sun, Jian-He; Wu, Nan; Li, Xing-Qi; Zhai, Suo-Qiang; Han, Dong-Yi; Young, Wie-Yen; Yang, Shi-Ming

来源：Journal of Nanoscience and Nanotechnology, 2010, 10(11): 7262-7265.

DOI：10.1166/jnn.2010.2822

• Math1 Gene
• Nanoparticles
• Quaternized Chitosan/Na-Carboxymethyl-beta-Cyclodextrin
• Cell Transfect

Mammalian cochlear hair cells don't regenerate naturally after injury, which usually leave permanent hearing loss. Math1 gene is a positive regulator of hair cell differentiation during cochlear development and was proved to be very critical in hair cell regeneration in deaf animals. Generating new cochlear hair cells by forced Math1 expression may be a cure for hearing loss. However, satisfying gene delivering vectors in gene therapy are not available. We combined quaternized chitosan (QCS) with Na-carboxymethyl-beta-cyclodextrin (CM-beta-CD) as novel non-viral vector, which adsorbs pRK5-Math1-EGFP perfectly at the mass ratio of 4:1. In vitro cell transfection can reach a 40% transfect efficiency and relatively low cytotoxity than liposomes. These results suggest that QCS/CM-beta-CD nanoparticle complexes could be a novel non-viral gene carrier in further clinical application.

• 出版日期2010-11
• 单位中国人民解放军总医院; 国家纳米科学中心