An efficient colorimetric biosensor for glucose based on peroxidase-like protein-Fe3O4 and glucose oxidase nanocomposites

作者：Liu Yan; Yuan Min; Qiao Longjiao; Guo Rong^*

来源：Biosensors and Bioelectronics, 2014, 52: 391-396.

DOI：10.1016/j.bios.2013.09.02

• Fe3O4 nanoparticles
• Protein
• Glucose
• Detection
• Peroxidase
• Enzyme immobilization

An efficient colorimetric biosensor for glucose based on peroxidase-like protein-Fe3O4 and glucose oxidase nanocomposites is reported in this work. Compared with bare MNPs, peroxidase-like casein-MNPs exhibit good catalytic properties, stability, dispersibility. Casein incorporated on MNPs notably improves the affinity toward both H2O2 and TMB, proved by variation in the determined kinetic parameters. As low as 0.2 mu M H2O2 can be detected with a linear range from 0.5 mu M to 200 mu M H2O2. More importantly, the casein/MNP nanocomposite was further used to immobilize GO(x) and to construct a glucose biosensor for the one-step determination of glucose. This method is simple, inexpensive, highly sensitive, and selective for glucose detection, with a detection limit of 1.

• 出版日期2014-2-15
• 单位扬州大学