Determination of naphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-018) in mouse blood and tissue after inhalation exposure to buzz%26apos; smoke by HPLC/MS/MS

作者：Poklis Justin L^*; Amira Dorra; Wise Laura E; Wiebelhaus Jason M; Haggerty Brenda J; Lichtman Aron H; Poklis Alphonse

来源：Biomedical Chromatography, 2012, 26(11): 1393-1398.

DOI：10.1002/bmc.2710

• JWH-018
• HPLC
• MS
• MS
• Herbal Incense product

The disposition of the cannabimimetic naphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-018) in mice following inhalation of the smoke of the herbal incense product (HIP) Buzz is presented. A high-pressure liquid chromatography with electrospray ionization triple quadrupole mass spectrometer (HPLC/MS/MS) method was validated for the analysis of JWH-018 in the specimens using deuterated ?9-tetrahydrocannabinol (d3-THC) as the internal standard. JWH-018 was isolated by cold acetonitrile liquidliquid extraction. Chromatographic separation was performed on a Zorbaz eclipse XDB-C18 column. The assay was linear from 1 to 1000?ng/mL. Six C57BL6 mice were sacrificed 20?min after exposure to the smoke of 200?mg Buzz containing 5.4% JWH-018. Specimen concentrations of JWH-018 were: blood, 54166?ng/mL (mean 82?+/-?42?ng/mL); brain, 316708?ng/g (mean 510?+/-?166?ng/g); and liver, 13703220?ng/mL (mean 1990 +/-?752?ng/mL). The mean blood to brain ratio for JWH-018 was 6.8 and ranged from 4.2 to 10.9. After exposure, the responses of the mice were consistent with cannabinoid receptor type 1 activity: body temperatures dropped 7.3?+/-?1.1?degrees C, and catalepsy, hyperreflexia, straub tail and ptosis were observed. The brain concentrations and physiological responses are consistent with the hypothesis that the behavioral effects of Buzz are attributable to JWH-018.

• 出版日期2012-11