A novel method for the rapid detection of post-translationally modified visinin-like protein 1 in rat models of brain injury

作者:Bradley-Whitman Melissa A.; Roberts Kelly N.; Abner Erin L.; Scheff Stephen W.; Lynn Bert C.; Lovell Mark A.*
来源:Brain Injury, 2018, 32(3): 363-380.
DOI:10.1080/02699052.2017.1418907

摘要

Background: Although elevated serum levels of visinin-like protein 1 (VILIP-1), a neuron-specific calcium sensor protein, are associated with ischaemic stroke, only a single study has evaluated VILIP-1 as a biomarker of traumatic brain injury (TBI). The current proof-of-concept study was designed to determine whether serum VILIP-1 levels increase post-injury in a well-characterized rat unilateral cortical contusion model.Methods: Lateral flow devices (LFDs) rapidly (<20min) detected trace serum levels (pg/mL) of VILIP-1 in a small input sample volume (10 mu L). Temporal profiles of serum levels at baseline and post-injury were measured in male Sprague Dawley rats subjected to very mild-, mild unilateral-cortical contusion, or naive surgery and in male Sprague Dawley rats following a diffuse TBI or sham surgery.Results: Mean serum levels were significantly elevated by 0.5h post-injury and remained so throughout the temporal profile compared with baseline in very mild and mild unilateral contusions but not in naive surgeries. Serum levels were also elevated in a small cohort of animals subjected to a diffuse TBI injury.Conclusions: Overall, the current study demonstrates that the novel LFD is a reliable and rapid point-of-care diagnostic for the detection and quantification of serum levels of UB-VILIP-1 in a clinically relevant time frame.

  • 出版日期2018