A label-free impedimetric immunosensor for direct determination of the textile dye Disperse Orange 1

作者：Yang Jing; da Rocha Carolina Gomes; Wang Shengfu; Pupim Ferreira Antonio Aparecido; Yamanaka Hideko^*

来源：Talanta, 2015, 142: 183-189.

DOI：10.1016/j.talanta.2015.04.042

• Disperse Orange 1
• Impedimetric immunosensor
• Textile dye
• Antibody immobilization

A strategy for a label-free impedimetric immunosensor is described for detection of the textile dye Disperse Orange 1(DO1). The compounds 1,12-diaminododecane (DADD) and then 1,7-diaminoheptane (DAH) were firstly successively grafted onto a glassy carbon electrode (GCE) surface by electro-oxidation of one amino group, while the other terminal amino group was modified with the antibody anti-DO1. The construction process of the immunosensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and capacitance measurements. The electron transfer resistance (R-ct) exhibited an effective response to the affinity between the immobilized antibody and the antigen in solution. The linear range for the target compound was from 5.0 nmol L-1 to 0.5 mu mol L-1 (R=0.9980), and the limit of detection (LOD) was 7.56 nmol L-1. The proposed impedimetric immunosensor has the advantages of simplicity, cost-effectiveness, and sensitivity.

• 出版日期2015-9-1
• 单位湖北大学; Univ Estadual Paulista