Dip-Pen Nanolithography on SiOx and Tissue-Derived Substrates: Comparison with Multiple Biological Inks

作者：Kramer Marcus A; Park Heyjin C; Ivanisevic Albena^*

来源：Scanning, 2010, 32(1): 30-34.

DOI：10.1002/sca.20160

• biomedical materials
• dip-pen lithography
• scanning probe microscopy
• tissue engineering
• biomimetic

There has been extensive interest in the micro and nanoscale manipulation of various substrates in the past few decades. One promising technique is dip-pen nanolithography which has shown the capability to pattern substrates of all forms including, tissue-derived substrates. Patterning of tissue-derived substrates is of particular interest, as it would facilitate studies into controlling cell morphology and cell substrate interaction. To expand the field into this area both peptides and bioactive collagen-binding peptide-linked biomolecules were patterned to the inner collagenous zone of the Bruch's membrane (BM). Collagen-binding peptide, and extra cellular matrix (ECM) proteins laminin and fibronectin were patterned on the BM and SiOx. The lithographic protocol was facilitated by Triton X-100 which was used to clean the tissue-derived construct after harvesting. This produced a collagen-exposed BM which was more hydrophilic (contact angle 67 degrees /- 8.49 degrees) surface compared with other cleaning methods but it maintained similar surface roughness (root-mean-square) 80 /- 18 nm and collagen exposure. This type of surface can be readily patterned with the chosen inks under lower humidity conditions. SCANNING 32: 30-34, 2010.

• 出版日期2010-2