Design and synthesis of a new coumarin-based %26apos;turn-on%26apos; fluorescent probe selective for Cu+2

作者：Garcia Beltran Olimpo^*; Mena Natalia; Friedrich Leidi C; Netto Ferreira Jose Carlos; Vargas Victor; Quina Frank H; Nunez Marco T; Cassels Bruce K

来源：Tetrahedron Letters, 2012, 53(39): 5280-5283.

DOI：10.1016/j.tetlet.2012.07.082

• Cu+2 ion
• Turn-on probes
• Coumarin Schiff base
• Fluorescence sensor

The novel coumarin-based %26apos;turn-on%26apos; fluorescent probe (E)-3-(2,5-dimethoxybenzylideneamino)-7-hydroxy-2H-chromen-2-one (MGM) was designed, synthesized, and characterized. This compound shows high selectivity for Cu+2, combined with a large fluorescence enhancement upon binding to Cu2+. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu+2 complex formed is 2:1. Preliminary studies employing epifluorescence microscopy demonstrated that Cu+2 could be imaged in human neuroblastoma SH-SY5Y cells treated with MGM.

• 出版日期2012-9-26