Use of EST-SSR markers for genetic characterisation of commercial watermelon varieties and hybrid seed purity testing

作者：Kwon Y S^*

来源：Seed Science and Technology, 2013, 41(2): 245-256.

EST-SSR markers were used to identify 36 candidate and registration watermelon varieties and 20 major commercial watermelon varieties, and to assess hybrid seed purity of a commercial hybrid watermelon variety known as 'Speedggul'. A set of 300 EST-SSR primer pairs were applied for fingerprinting the watermelon varieties. Nine markers showed hyper-variability and were able to differentiate 56 genotypes, representing the genetic variation within varietal groups. Cluster analysis and Jaccard's distance coefficients using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) categorised varieties into two major groups corresponding to morphological traits. To evaluate the use of EST-SSR marker analysis for seed purity testing of F-1 hybrid watermelon Speedggul, the results of marker analysis were compared with the results of a grow-out test. The F-1 purity through grow-out test was in accordance with molecular marker analysis. These markers will be useful for fingerprinting watermelon varieties and help private seed companies to improve watermelon seed purity.

• 出版日期2013