Ascorbic Acid Synergistically Potentiates Phloxine B-induced Photocytotoxicity in Human Acute Promyelocytic Leukemia Cells

作者：Qi, Hang; Wu, Qian; Abe, Naomi; Saiki, Shunya; Zhu, Beiwei; Murata, Yoshiyuki; Nakamura, Yoshimasa^*

来源：Journal of Biochemical and Molecular Toxicology, 2014, 28(4): 167-173.

DOI：10.1002/jbt.21549

• Ascorbic Acid
• Phloxine B
• Photocytotoxicity
• Hydrogen Peroxide
• HL-60 Cells

Ascorbic acid (AsA) is known as an antioxidant but concomitantly possesses a pro-oxidant property. Because the impact of AsA on photodynamic therapy response is unclear, we investigated the effect of AsA on photocytotoxicity induced by phloxine B in human acute promyelocytic leukemia HL-60 cells. AsA synergistically enhanced phloxine B-induced photocytotoxic effects, including inhibition of cell proliferation, DNA ladder formation, and caspase-3 activation, whereas AsA itself showed no photocytotoxicity. AsA also enhanced the consumption of the reduced glutathione level compared with the cells treated with phloxine B alone under the light condition. Combination of AsA with phloxine B under the light condition enhanced the phosphorylation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase (MAPK). These effects were completely cancelled by catalase. These results suggest that AsA synergistically enhances phloxine B-induced photocytotoxicity, possibly through the extracellular oxidative stress-dependent MAPK pathway activation.

• 出版日期2014-4
• 单位大连工业大学