Decreased Expression of the Epithelial Ca2+ Channel TRPV5 and TRPV6 in Human Renal Cell Carcinoma Associated With Vitamin D Receptor

作者：Wu, Yongyang; Miyamoto, Tatsuya; Li, Kai; Nakagomi, Hiroshi; Sawada, Norifumi; Kira, Satoru; Kobayashi, Hideki; Zakohji, Hidenori; Tsuchida, Takayuki; Fukazawa, Mizuya; Araki, Isao; Takeda, Masayuki^*

来源：Journal of Urology, 2011, 186(6): 2419-2425.

DOI：10.1016/j.juro.2011.07.086

• kidney
• carcinoma, renal cell
• receptors, calcitriol
• TRPV5 protein, human
• TRPV6 protein, human

Purpose: We investigated the expression of epithelial Ca2+ channel TRPV (transient receptor potential vanilloid subfamily) 5 and 6, and vitamin D receptor in primary human renal cell carcinoma and benign peritumor tissues, and assessed the possible association between TRPV5/6 and vitamin D receptor expression.
Materials and Methods: Fresh-frozen primary tumor and peritumor tissues from 27 patients diagnosed with renal cell carcinoma were analyzed for TRPV5/6 and vitamin D receptor expression by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry.
Results: Quantitative reverse transcriptase-polymerase chain reaction revealed that TRPV5/6 and vitamin D receptor expression was decreased 38.11, 4.44 and 3.20 times in renal cell carcinoma vs normal kidney tissue (p = 0.012, 0.002 and 0.020, respectively). Relatively higher expression was noted for chromophobe renal cell carcinoma than for the other renal cell carcinoma subtypes. Vitamin D receptor mRNA expression significantly correlated with that of TRPV6 (r = 0.508, p = 0.007) and TRPV5 (r = 0.697, p = 0.032) in renal cell carcinoma. Western blot showed results similar to those of reverse transcriptase-polymerase chain reaction. Different expression was detected between kidney and renal cell carcinoma tissue. Immunohistochemical analysis verified strong detection of TRPV5/6 and vitamin D receptor in distal nephrons but demonstrated weak or no immunostaining much more often in renal cell carcinoma.
Conclusions: Decreased TRPV5/V6 expression was noted in renal cell carcinoma, which correlated with vitamin D receptor. Different expression was also detected among the different renal cell carcinoma histopathological subtypes. Our observations suggest that altered vitamin D receptor expression may be associated with renal cell carcinoma carcinogenesis via TRPV5/6.

• 出版日期2011-12
• 单位福建医科大学