2D-immunoblotting analysis of Sporothrix schenckii cell wall

作者：Ruiz Baca Estela^*; Mora Montes Hector M; Lopez Romero Everardo; Toriello Conchita; Mojica Marin Virgilio; Urtiz Estrada Norma

来源：Memorias do Instituto Oswaldo Cruz, 2011, 106(2): 248-250.

• sporotrichosis
• Sporothrix schenckii
• cell wall
• glycoproteins
• 2D-immunoblotting analysis

We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.

• 出版日期2011-3