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摘要
Background: Klebsiella species are important opportunistic pathogens causing a variety of infections, especially in hospitalized and immunocompromised patients.
Objectives: To investigate the clinical prevalence of five different Klebsiella species (K. pneumoniae, K. ornithinolytica, K. oxytoca, K. terrigena, and K. rhinoscleromatis) including antibiations (trimethoprim-sulfamethoxazole, ampicillin-sulotic resistance profiles using six different antibiotics and combinbactam, imipenem, piperacillin-tazobactam, ciprofloxacin, ceftizoxime).
Methods: Resistance of Klebsiella spp. including multidrug resistant (MDR) strains was determined by using a Kirby-Bauer disk diffusion method and genotypical analysis was performed by enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR).
Results: Urine samples and the urology service unit were the most common sources of K. ornithinolytica, K. pneumoniae, and K. terrigena strains. The greatest drug resistance was observed against trimethoprim-sulfamethoxazole (88%), the least resistance was observed against imipenem (12%). Apart from these, 11 different antibiotypes were generated and antibiotype AI (resistant only to trimethoprim-sulfamethoxazole) was the most frequently observed (40%). MDR profiles of Klebsiella spp. were also investigated and 25% of all Klebsiella spp. strains were found to be MDR; and 65% of these were isolated from urine samples. MDR strains were mostly found to be K. ornithinolytica (35%) followed by K. pneumoniae (29%). Genotyping was performed by using ERIC PCR and Klebsiella spp. strains were grouped in 23 genotypes with a similarity coefficient of 70%.
Conclusions: Antibiotyping and antibiotype profiles may provide valuable information for hospitalized patients that could identify problem spots and allow evidence-based provision of preventive measures against nosocomial emergence of infections with new MDR strains.
- 出版日期2016-2
