Hydrophilic interaction liquid chromatography-tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

作者：Xiong, Zhili; Zhang, Yi; Qin, Feng; Qin, Ting; Yang, Shuyan; Li, Famei^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(23): 2111-2116.

DOI：10.1016/j.jchromb.2010.06.024

• Adefovir
• HILIC-MS/MS
• Human plasma
• Pharmacokinetic study

A selective, rapid and sensitive hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was developed for the first time to determine adefovir in human plasma and applied to a pharmacokinetic study. Plasma samples were prepared by protein precipitation with methanol followed by a further cleaning using dichloromethane. The chromatographic separation was carried out on an ACQUITY UPLC (TM) BEH HILIC column with the mobile phase of methanol-water-formic acid (85:15:0.2, v/v/v). The detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. The method was rapid with a run time of 3 min per sample. The linear calibration curves were obtained in the concentration range of 1.02-102 ng/mL (r(2) >= 0.99) with the lower limit of quantification (LLOQ) of 1.02 ng/mL. The intra- and inter-clay precision (relative standard deviation, R.S.D.) values were below 12% and the accuracy (relative error. RE.) was from 0.6% to 3.2% at all quality control (QC) levels. The method was applicable to clinical pharmacokinetic study of adefovir in healthy volunteers after oral administration of adefovir dipivoxil tablet.

• 出版日期2010-8-1
• 单位沈阳药科大学