A Snapshot of the Physical and Functional Wiring of the Eps15 Homology Domain Network in the Nematode

作者：Tsushima Hanako; Malabarba Maria Grazia; Confalonieri Stefano; Senic Matuglia Francesca; Verhoef Lisette G G C; Bartocci Cristina; D' Ario Giovanni; Cocito Andrea; Di Fiore Pier Paolo^*; Salcini Anna Elisabetta

来源：PLos One, 2013, 8(2): e56383.

DOI：10.1371/journal.pone.0056383

Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of %26quot;territories%26quot; of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

• 出版日期2013-2-12