Anti-interleukin-1 beta antibody prevents the occurrence of repeated restraint stress-induced alterations in synaptic transmission and long-term potentiation in the rat frontal cortex

作者：Bobula Bartosz^*; Sowa Joanna; Hess Grzegorz

来源：Pharmacological Reports, 2015, 67(1): 123-128.

DOI：10.1016/j.pharep.2014.08.011

• Frontal cortex
• Restraint stress
• Interleukin-1 beta
• LTP

Background: The mechanisms of the influence of prolonged stress on glutamatergic transmission and synaptic plasticity in the cerebral cortex remain poorly understood. The purpose of this study was to determine an involvement of interleukin-1 beta (IL-1 beta) in the effects of repeated restraint stress on excitatory synaptic transmission and long-term potentiation (LTP) in the rat frontal cortex.
Methods: The effects of restraint stress lasting for 10 min, repeated twice daily for 3 consecutive days were studied ex vivo in the rat frontal cortex slices prepared 24 h after the last stress session. Rats received intraperitoneal injections of interleukin-1 beta antibody. In a separate experimental group, rats received injections of IL-1 beta. Field potentials were recorded in the cortical layer II/III.
Results: In slices originating from stressed animals, the amplitude of field potentials was increased. Consistent with the previous studies, restraint stress resulted in a reduced magnitude of LTP. Similar effects were evident after administration of IL-1 beta. Stress-induced modifications of the glutamatergic transmission and synaptic plasticity were prevented by interleukin-1 beta antibody, which was administered 15 min before each restraint session.
Conclusions: These data point to an involvement of peripherally produced IL-1 beta in mediating the influence of repeated restraint stress on the functions of the frontal cortex.

• 出版日期2015-2